Application of liquid chromatography-mass spectrometry for determination of indole and 3-methylindole in porcine adipose tissue

Abstract

A new method is described for simultaneous determination of 3-methylindole (3MI) and indole in porcine adipose tissue. Sample preparation included liquid-liquid extraction with n-hexane and 75% aqueous acetonitrile. The acetonitrile extracts were analysed by liquid chromatography-mass spectrometry (LC-MS) using atmospheric pressure chemical ionization (APCI) with selective ion monitoring of protonated ions [M + H](+). This method showed excellent linearit over the concentration range tested (from 2 to 500 ng mL(-1) for 3MI and from 1 to 500 ng mL(-1) for indole) and good accuracy (recovery of 92 +/- 10% for 3MI and 91 +/- 10% for indole). This new LC-MS method was compared with traditional calorimetric method for 3MI equivalent. Additionally, the correlation between 3MI concentrations in adipose tissue and plasma was studied. The described LC-MS method can be used to quantify 3MI and indole in porcine adipose tissue in various endocrinological or meat science studies

Keywords

Entire male pigs; boar -taint; skatole; androstenone; plasma; feces; HPLC

Published in

Chromatographia
2006, volume: 64, number: 7-8, pages: 435-439

SLU Authors

• Zamaratskaia, Galia

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Jastrebova, Jelena

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science

Permanent link to this page (URI)

https://res.slu.se/id/publ/9238