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Forskningsartikel2017Vetenskapligt granskad

Cytoprotective Encapsulation of Individual Jurkat T Cells within Durable TiO2 Shells for T-Cell Therapy

Youn, Wongu; Ko, Eun Hyea; Kim, Mi-Hee; Park, Matthew; Hong, Daewha; Seisenbaeva, Gulaim A.; Kessler, Vadim G.; Choi, Insung S.

Sammanfattning

Lymphocytes, such as T cells and natural killer (NK) cells, have therapeutic promise in adoptive cell transfer (ACT) therapy, where the cells are activated and expanded in vitro and then infused into a patient. However, the in vitro preservation of labile lymphocytes during transfer, manipulation, and storage has been one of the bottlenecks in the development and commercialization of therapeutic lymphocytes. Herein, we suggest a cell-in-shell (or artificial spore) strategy to enhance the cell viability in the practical settings, while maintaining biological activities for therapeutic efficacy. A durable titanium oxide (TiO2) shell is formed on individual Jurkat T cells, and the CD3 and other antigens on cell surfaces remain accessible to the antibodies. Interleukin-2 (IL-2) secretion is also not hampered by the shell formation. This work suggests a chemical toolbox for effectively preserving lymphocytes in vitro and developing the lymphocyte-based cancer immunotherapy.

Nyckelord

artificial spores; cytoprotection; immunology; mineralization; titanates

Publicerad i

Angewandte Chemie International Edition
2017, Volym: 56, nummer: 36, sidor: 10702-10706
Utgivare: WILEY-V C H VERLAG GMBH

      SLU författare

      • Kessler, Vadim

        • Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet

      Globala målen

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      UKÄ forskningsämne

      Organisk kemi

      Publikationens identifierare

      DOI: https://doi.org/10.1002/anie.201703886

      Permanent länk till denna sida (URI)

      https://res.slu.se/id/publ/92712