Oskarsson, Agneta
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Research article2017Peer reviewedOpen access
Oskarsson, A.; Yagdiran, Y.; Nazemi, S.; Tallkvist, J.; Knight, C. H.
Mastitis is the most common disease in dairy herds worldwide and is often caused by Staphylococcus aureus. Little is known about the effect of mastitis on transporters in the mammary gland and the effect on transporter-mediated secretion of drugs into milk. We studied gene expressions of ATP-binding cassette and solute carrier transporters in S. aureus-infected mammary glands of mice. On d 7 of lactation, NMRI mice were inoculated with 1,000 cfu of S. aureus in 2 mammary glands and with a saline vehicle in 2 control glands. Gene expression of the transporters, Bcrp, Mdr1, Mrp1, Oatp1a5, Octn1, and Oct1, and of Csn2, the gene encoding O-casein, were determined in mammary glands at 72 h after treatment. As biomarkers of the inflammatory response gene, expressions of the cytokines Il6, Tnf alpha, and the chemokine Cxc12 were measured. Despite a high individual variation between the 6 animals, some characteristic patterns were evident. The 3 inflammatory biomarkers were upregulated in all animals; Csn2 was downregulated compared with controls in all animals, although not statistically significantly. Both Mrp1 and Oatp1a5 were statistically significantly upregulated and Bcrp was downregulated. Gene expression of Bcrp followed the expression of Csn2 in each of the animals, indicating a possible co-regulation. The findings demonstrate that S. aureus infection has an effect on expression of drug transporters in the mammary gland, which may affect secretion of drugs into milk and efficacy of drug therapy.
mastitis; drug transporters; mammary gland; Staphylococcus aureus
Journal of Dairy Science
2017, Volume: 100, number: 3, pages: 2375-2380
SDG3 Good health and well-being
Animal and Dairy Science
Pharmacology and Toxicology
Public Health, Global Health, Social Medicine and Epidemiology
DOI: https://doi.org/10.3168/jds.2016-11650
https://res.slu.se/id/publ/92791