Skip to main content
SLU:s publikationsdatabas (SLUpub)

Forskningsartikel2017Vetenskapligt granskad

Metabolic changes in serum metabolome in response to a meal

Shrestha, Aahana; Mullner, Elisabeth; Poutanen, Kaisa; Mykkanen, Hannu; Moazzami, Ali A.


The change in serum metabolic response from fasting state to postprandial state provides novel insights into the impact of a single meal on human metabolism. Therefore, this study explored changes in serum metabolite profile after a single meal.Nineteen healthy postmenopausal women with normal glucose tolerance participated in the study. They received a meal consisting of refined wheat bread (50 g carbohydrates, 9 g protein, 4.2 g fat and 2.7 g dietary fibre), 40 g cucumber and 300 mL noncaloric orange drink. Blood samples were collected at fasting and five postprandial time points. Metabolic profile was measured by nuclear magnetic resonance and targeted liquid chromatography-mass spectrometry. Changes over time were assessed with multivariate models and ANOVA, with baseline as control.The metabolomic analyses demonstrated alterations in phospholipids, amino acids and their breakdown products, glycolytic products, acylcarnitines and ketone bodies after a single meal. More specifically, phosphatidylcholines, lysophosphatidylcholines and citrate displayed an overall declining pattern, while leucine, isoleucine, methionine and succinate increased initially but declined thereafter. A sharp decline in acylcarnitines and ketone bodies and increase in glycolytic products postprandially suggest a switch in the body's energy source from beta-oxidation to glycolysis. Moreover, individuals with relatively high postprandial insulin responses generated a higher postprandial leucine responses compared to participants with lower insulin responses.The study demonstrated complex changes from catabolic to anabolic metabolism after a meal and indicated that the extent of postprandial responses is different between individuals with high and low insulin response.


Metabolomics; Postprandial changes; Insulin; Amino acid; Acylcarnitine; Glycolytic products; Phosphatidylcholine

Publicerad i

European Journal of Nutrition
2017, Volym: 56, nummer: 2, sidor: 671-681

      SLU författare

    • Shrestha, Aahana

      • Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
      • Müllner, Elisabeth

        • Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
        • Moazzami, Ali

          • Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet

        Globala målen

        SDG3 God hälsa och välbefinnande
        SDG5 Jämställdhet

        UKÄ forskningsämne


        Publikationens identifierare


        Permanent länk till denna sida (URI)