Abstract

Fourier transform infrared (FT-IR) spectroscopy is a fast, sensitive, inexpensive, and nondestructive technique for chemical profiling of plant materials. In this chapter we discuss the instrumental setup, the basic principles of analysis, and the possibilities for and limitations of obtaining qualitative and semiquantitative information by FT-IR spectroscopy. We provide detailed protocols for four fully customizable techniques: (1) Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS): a sensitive and high-throughput technique for powders; (2) attenuated total reflectance (ATR) spectroscopy: a technique that requires no sample preparation and can be used for solid samples as well as for cell cultures; (3) microspectroscopy using a single element (SE) detector: a technique used for analyzing sections at low spatial resolution; and (4) microspectroscopy using a focal plane array (FPA) detector: a technique for rapid chemical profiling of plant sections at cellular resolution. Sample preparation, measurement, and data analysis steps are listed for each of the techniques to help the user collect the best quality spectra and prepare them for subsequent multivariate analysis.

Keywords

Fourier transform infrared spectroscopy; Methods; Microspectroscopy; Chemical composition; Multivariate analysis; Plant; Attenuated total reflectance; Diffuse reflectance; Focal plane array detector

Published in

Methods in Molecular Biology
2014, Volume: 1062, number: 1062, pages: 317-352
Title: Arabidopsis Protocols
ISBN: 978-1-62703-579-8, eISBN: 978-1-62703-580-4
Publisher: Humana Press

SLU Authors

• Sundberg, Björn

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1007/978-1-62703-580-4_18

Permanent link to this page (URI)

https://res.slu.se/id/publ/95240