Expression of nirK and nirS genes in two strains of Pseudomonas stutzeri harbouring both types of NO-forming nitrite reductases

Sammanfattning

Reduction of nitrite to nitric oxide in denitrification is catalysed by two different nitrite reductases, encoded by nirS or nirK. Long considered mutually exclusive and functionally redundant in denitrifying bacteria, we show expression of both genes co-occurring in Pseudomonas stutzeri. The differential expression patterns between strain AN10 and JM300 in relation to oxygen and nitrate and their different denitrification phenotypes, with AN10 reducing nitrate more rapidly and accumulating nitrite, suggest that nirS and nirK can have different roles. Dissimilar gene arrangements and transcription factors in the nir gene neighbourhoods could explain the observed differences in gene expression and denitrification activity. (C) 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Nyckelord

AN10; Denitrification; Gene arrangement; JM300; Phenotype

Publicerad i

Research in Microbiology
2018, Volym: 169, nummer: 6, sidor: 343-347 Utgivare: ELSEVIER SCIENCE BV

SLU författare

• Wittorf, Lea

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

• Jones, Christopher

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

• Bonilla Rosso, German

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

• Hallin, Sara

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Mikrobiologi


Publikationens identifierare

DOI: https://doi.org/10.1016/j.resmic.2018.04.010

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/96221