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Forskningsartikel2002Vetenskapligt granskad

Muscarinic receptors in equine airways

Torneke, K; Ingvast-Larsson, C; Bostrom, A; Appelgren, LE


The distribution of muscarinic receptors in equine airways was investigated using autoradiography. Frozen sections of tissue from six different levels in the bronchial tree, from the trachea to the distal bronchioles, were incubated in vitro with 1.5 nmol/L of the muscarinic receptor antagonist 1-[N-methyl-H-3]scopolamine methyl chloride (H-3-NMS). In addition, the subtype pattern of muscarinic receptors was investigated in equine tracheal smooth muscle using radioligand binding with methoctramine, tripinamide, 4-DAMP-methiodide and pirenzipine as competitors against the binding of 1.3 nmol/L H-3-NMS. The autoradiograms showed specific labelling indicating a high density of muscarinic receptors in smooth-muscle tissue in all levels of the airway tree investigated. Besides muscle tissue, subepithelial glands were the only structures specifically labelled. The dominating subtypes in tracheal smooth muscle investigated with radioligand binding studies were found to be M-2 and M-4, as both methoctramine (pK(d) = 8.5) and tripinamide (pK(d) = 8.6 and 6.7 for two different sites) showed high affinity. The density of the M-3-muscarinic receptor subtype was low, but this subtype could be detected with statistical significance when methoctramine was used as the competitor against H-3-NMS binding.


antagonists; autoradiography; bronchi; bronchioles; horse; muscarinic receptors; trachea

Publicerad i

Veterinary Research Communications
2002, Volym: 26, nummer: 8, sidor: 637-650

      SLU författare

    • Törneke, Karolina

      • Institutionen för farmakologi och toxikologi, Sveriges lantbruksuniversitet
      • Ingvast Larsson, Carina

        • Institutionen för farmakologi och toxikologi, Sveriges lantbruksuniversitet
        • Boström, Agneta

          • Institutionen för farmakologi och toxikologi, Sveriges lantbruksuniversitet
        • UKÄ forskningsämne


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