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Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development

Jagarlamudi, K. K.; Wang, L.; Eriksson, S.


In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure of CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. In cell extracts, TK1 levels increased twofold with 1 mu M Dox in both cell lines, while the TK1 levels in the culture media increased with 5 and 10 mu M of Dox only in case of CEM cells. In conclusion, this study reveals that the TK 210 ELISA can measure changes in intra- and extracellular TK1 levels apparently related to the mechanism of cytotoxicity of anti-cancer agents.


Doxorubicin; TK 210 ELISA; Thymidine kinase 1

Publicerad i

Nucleosides, Nucleotides and Nucleic Acids
2018, Volym: 37, nummer: 12, sidor: 679-686