1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium

Abstract

A glycoside hydrolase family 45 (GH45) enzyme from the white-rot basidiomycete fungus Phanerochaete chrysosporium (PcCel45A) was expressed in Pichia pastoris with C-13 and N-15 labelling. A nearly complete assignment of H-1, C-13 and 15N backbone resonances was obtained, as well as the secondary structure prediction based on the assigned chemical shifts using the TALOS-N software. The predicted secondary structure was almost identical to previously published crystal structures of the same enzyme, except for differences in the termini of the sequence. This is the first NMR study using an isotopically labelled GH45 enzyme.

Keywords

PcCel45A; Cel45A; GH45; Endoglucanase; NMR

Published in

Biomolecular NMR Assignments
2024, volume: 18, number: 2, pages: 153-157
Publisher: SPRINGER

SLU Authors

• Okmane, Laura

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Sandgren, Mats

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Ståhlberg, Jerry

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Nestor, Gustav

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Structural Biology
Molecular Biology
Biochemistry

Publication identifier

• DOI: https://doi.org/10.1007/s12104-024-10182-6

Permanent link to this page (URI)

https://res.slu.se/id/publ/131086