Hellman, Maria
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Research article2011Peer reviewed
Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates
Hellman, Maria; Berg, Jeanette; Brandt, Kristian K.; Hallin, Sara
Abstract
Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU. (C) 2011 Elsevier B.V. All rights reserved.
Keywords
Active bacteria; BrdU incorporation; Replicating bacteria; Soil bacteria; Thymidine analog
Published in
Journal of Microbiological Methods
2011, volume: 86, number: 3, pages: 376-378
Publisher: ELSEVIER SCIENCE BV
SLU Authors
Hallin, Sara
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Agricultural Science
Fish and Aquacultural Science
Environmental Sciences related to Agriculture and Land-use
Publication identifier
- DOI: https://doi.org/10.1016/j.mimet.2011.05.020
Permanent link to this page (URI)
https://res.slu.se/id/publ/46481