Elander, Pernilla
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Research article2021Peer reviewedOpen access
Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
Gutierrez-Beltran, Emilio; Elander, Pernilla H.; Dalman, Kerstin; Dayhoff, Guy W.; Moschou, Panagiotis N.; Uversky, Vladimir N.; Crespo, Jose L.; Bozhkov, Peter, V
Abstract
Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein-protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.
Keywords
heat stress; intrinsically disordered regions; SnRK1; SNF1; AMPK; stress granules; tudor staphylococcal nuclease
Published in
The Embo Journal
2021, volume: 40, number: 17, article number: e105043
Publisher: WILEY
SLU Authors
Dalman, Kerstin
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Moschou, Panagiotis Nikolaou
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Foundation for Research and Technology - Hellas (FORTH)
- University of Crete
- Department of Plant Biology, Swedish University of Agricultural Sciences
Bozhkov, Peter
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Biochemistry and Molecular Biology
Publication identifier
- DOI: https://doi.org/10.15252/embj.2020105043
Permanent link to this page (URI)
https://res.slu.se/id/publ/113227