Eriksson, Bengt
- Institutionen för obstetrik och gynekologi, Sveriges lantbruksuniversitet
Sammanfattning
The world’s pig population is consistently being upgraded through the international trade of superior genetics. The two major systems that are used for this purpose are the transport of live animals and the export of frozen boar semen. The main limiting factors for a wider use of frozen-thawed (FT) boar semen are low fertility levels of FT in comparison with liquid semen, and between-boar variation in freezing success. Consequently, there is a need for improved boar semen freezing methods. The main objective of this thesis was to develop a method for commercial freezing of boar semen, and the study of the effect of different factors on boar sperm survival in vitro and fertility after freezing-thawing in large, one-AI-dose containers. Semen was split-sample frozen in 5 mL Maxi-straws and in Cochette plastic bags. A computer-assisted sperm analyser (CASA) was used to evaluate sperm motility, while plasma membrane integrity (PMI) was assessed with fluorescent dyes. The fertilising capacity of the semen frozen in the two containers was assessed by inseminating (AI) gilts. The Cochettes yielded a significantly (W0.05) higher motility post-thaw, but the opposite was observed for PMI. No difference in fertilising capacity (pregnancy rate and number of viable embryos) could be seen. It was concluded that though proven feasible, the Cochette is not a suitable container for the commercial freezing of boar semen. The effects of different freezing and thawing rates on the post-thaw motility and PMI of boar spermatozoa, processed in either Maxi-straws or flat plastic packages called FlatPacks were studied. Values for percentage motile spermatozoa, sperm velocity and lateral head displacement, were significantly higher for samples frozen in FlatPacks than for those frozen in Maxi-straws (P
Nyckelord
semen; cooling; cryopreservation; thawing rate; freezing packages; freezing rate; holding time; in vitro oocyte penetration ability; motility; plasma membrane integrity; field fertility; boar
Publicerad i
Acta Universitatis Agriculturae Sueciae. Veterinaria
2000, nummer: 91
Utgivare: Swedish University of Agricultural Sciences
SLU författare
UKÄ forskningsämne
Klinisk vetenskap
Publikationens identifierare
- ISBN: 91-576-5903-6
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/107416