Cytochemical and immunocytochemical characterization of wood decayed by the white rot fungus Pycnoporus sanguineus II. Degradation of lignin and non-cellulosic polysaccharides in European ash wood

Sammanfattning

Degradation of lignin and non-cellulosic polysaccharides (pectins and hemicelluloses) by the white rot fungus Pycnoporus sanguineus were investigated in European ash wood (hardwood) using transmission electron microscopy coupled with immunocytochemistry. Lignin was preferentially removed in secondary cell walls of ash xylem cells including fibers, vessel and (ray/axial) parenchyma cells prior to degradation of hemicelluloses (i.e. selective decay). Concomitantly, degradation of cell walls occurred by progressive thinning from the lumen surface outwards similar to simultaneous decay. Preferential degradation of middle lamellae before complete degradation of secondary cell walls (i.e. a typical feature of selective decay) was only observed in bi-/triseriate rays by initial attack from pit regions. Vessel and parenchyma cells showed greater resistance to decay than fibers with narrower decay zone produced. A comparison of decay between ash and spruce wood (Part I, softwood) demonstrated that P.sanguineus produces selective decay in both hard- and softwoods but more typical micromorphological features of selective decay are produced in spruce tracheids than ash fibers. This study highlights that degradation of lignin proceeds hemicelluloses in wood cell walls attacked by the selective white rot fungus P.sanguineus.

Nyckelord

European ash (hardwood); Hemicelluloses; Immunocytochemistry; Lignin; Pycnoporus sanguineus; Selective decay

Publicerad i

International Biodeterioration and Biodegradation
2015, volym: 105, sidor: 41-50

SLU författare

• Kim, Jongsik

  • Institutionen för skogens produkter, Sveriges lantbruksuniversitet
    

• Gao, Jie

  • Institutionen för skogens produkter, Sveriges lantbruksuniversitet
    

• Daniel, Geoffrey

  • Institutionen för skogens produkter, Sveriges lantbruksuniversitet
    

Associerade SLU-program

SLU Nätverk växtskydd

UKÄ forskningsämne

Trävetenskap
Skogsvetenskap

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.ibiod.2015.08.009

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/68595