Transmission of double-stranded RNA in Heterobasidion annosum

Sammanfattning

Transmission of dsRNA viruses between homo- and heterokaryotic mycelia paired on agar plates and into conidia has been studied in Heterobasidion annosum. Horizontal transmission of dsRNA occurred between both homo- and heterokaryotic isolates, as well as between isolates belonging to different intersterility groups. The proportions of vertical transmission into conidia were 3% and 55%, respectively, for the two isolates included in the study. RT-PCR of dsRNA and PCR-RFLP of mitochondrial markers were used to confirm transmission of dsRNA between the cytoplasms of different mycelia. The identity of nuclei and nuclear migration during experiments were verified using PCR-RFLP of several nuclear markers. (C) 2002 Elsevier Science (USA). All rights reserved.

Nyckelord

Heterobasidion annostan; mycovirus; transmission; dsRNA; mitochondrial markers; nuclear markers; basidiomycete; plant pathogen; horizontal transfer; vertical transfer

Publicerad i

Fungal Genetics and Biology
2002, volym: 36, nummer: 2, sidor: 147-154
Utgivare: ACADEMIC PRESS INC ELSEVIER SCIENCE

SLU författare

• Ihrmark, Katarina

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

• Stenström, Elna

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

• Stenlid, Jan

  • Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Mikrobiologi
Annan biologi

Publikationens identifierare

• DOI: https://doi.org/10.1016/S1087-1845(02)00011-7

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/80793