Dotsenko, Gleb
- Росси́йская акаде́мия нау́к
Sammanfattning
Three approaches for enrichment of Penicillium verruculosum cellulose multienzyme cocktails with a heterologously expressed Aspergillus niger beta-glucosidase (AnBGL) for boosting the hydrolytic performance of cellulases were tested. The best boosting effects (73-113%) on glucose yield in 72-h hydrolysis of milled aspen wood and Avicel provided the enzyme preparation obtained by cofermentation of two P. verruculosum strains, one of which was a basic high-cellulase strain, while the other was capable to produce the heterologously expressed AnBGL (up to 80% of the total protein when used in a separate fermentation). Two other approaches, based on individual fermentations of P. verruculosum strains, carrying the heterologous AnBGL under the control of either a strong cbh1 gene promoter or a weaker hist4 gene promoter, also provided notable boosting effects, although they were less pronounced than in the first mentioned case. (C) 2015 Elsevier Ltd. All rights reserved.
Nyckelord
beta-Glucosidase; Cellulose; Heterologous expression; Penicillium verruculosum
Publicerad i
Process Biochemistry
2015, volym: 50, nummer: 8, sidor: 1258-1263
SLU författare
UKÄ forskningsämne
Biokatalys och enzymteknik
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.procbio.2015.05.008
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/84293