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Sammanfattning

A 25AD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg2+-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligorners. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein.

Nyckelord

potato virus X; movement protein; oligomerization; yeast two-hybrid system; glutaraldebyde

Publicerad i

Биохимия / Biochemistry
2008, volym: 73, nummer: 1, sidor: 50-55

SLU författare

UKÄ forskningsämne

Molekylärbiologi
Botanik
Biokemi

Publikationens identifierare

  • DOI: https://doi.org/10.1134/S0006297908010070

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/91715