Sammanfattning

In a wide range of salt concentrations, 10–30 mM phosphate buffer containing up to 0.5 M Li₂SO₄ and 300 mM NaCl, 7.5 mM Mg²⁺, pH 5.5–7.5, a mixture of the 16 mer and the 25 mer RNA strands does not form a hammerhead in any amount detectable by NMR at 600 MHz. The imino-, amino-, aromatic- and anomeric protons in the NMR spectra of both the 16 mer and the 25 mer RNA have been assigned separately. Both the 16 mer and the 25 mer RNA both take up very stable hairpin structures, and when mixed together there is no major change of conformation in neither oligo-RNA.

Publicerad i

Nucleosides, Nucleotides and Nucleic Acids
2001, Volym: 20, nummer: 4-7, sidor: 1219-1223

UKÄ forskningsämne

Organisk kemi


Publikationens identifierare

DOI: https://doi.org/10.1081/NCN-100002522

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/108060