Sammanfattning

Aquatic pollution with faecal bacteria and subsequent consumption of contaminated water or food is a worldwide issue that causes severe health effects (e.g. meningitis, salmonellosis, dysentery). In addition, the excessive use of antibiotics in animal husbandry and human medicine has enhanced the selective pressure on pathogenic bacteria, further increasing human health risks and detrimental effects on natural microbial communities. This urges the need to monitor faecal contamination using a time-integrated approach, as grab water samples can miss pathogen peaks. We tested the ability of zebra mussels (Dreissena polymorpha) to take up and depurate faecal indicator bacteria such as Escherichia coli and intestinal enterococci. Furthermore, we quantified the frequency of antibiotic resistant bacteria in water and mussels both in controlled laboratory tests and under in situ conditions downstream of a sewage treatment plant (STP). Laboratory results show that bacterial indicators in mussels were 132 times higher than their concentration in water, and that mussels retained bacteria up to 2 days after pulse exposure. Field results show decreasing bacterial concentrations in both water and mussels downstream the STP, with maximum E. coli concentrations ranging 173-9 cfu mL(-1) in water and 2970-330 cfu g(-1) in mussels. Similarly, enterococci ranged 59-4 cfu mL(-1) and 1450-240 cfu g(-1) in water and mussels, respectively. High proportions of antibiotic resistant E. coli were found in mussels (72%) and water (65%), and slightly lower proportion of resistant enterococci was found in mussels (47%) and in water (34%). Moreover, 33% of the bacteria isolated from mussels were resistant to multiple antibiotics, which emphasizes that resistance is a common feature in surface waters and highlights the need for safe water management. Our results show that zebra mussels provide an efficient, time-integrating tool for quantifying faecal indicators, including resistant and multidrug resistant bacteria. (C) 2018 The Authors. Published by Elsevier B.V.

Nyckelord

Faecal; Pathogen; In situ; Escherichia coli; Enterococcus; Salmonella

Publicerad i

Science of the Total Environment
2019, Volym: 650, nummer: Part 2, sidor: 2141-2149
Utgivare: ELSEVIER SCIENCE BV

SLU författare

• Bighiu, Maria

  • Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
    

• Norman Haldén, Anna

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

• Goedkoop, Willem

  • Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
    

Associerade SLU-program

AMR: Bakterier


Globala målen

SDG6 Säkerställa tillgången till och en hållbar förvaltning av vatten och sanitet för alla
SDG3 Säkerställa hälsosamma liv och främja välbefinnande för alla i alla åldrar
SDG2 Avskaffa hunger, uppnå tryggad livsmedelsförsörjning och förbättrad nutrition samt främja ett hållbart jordbruk


UKÄ forskningsämne

Miljövetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.scitotenv.2018.09.314

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/96976