Andersson, Kristina
- Department of Applied Animal Science and Welfare, Swedish University of Agricultural Sciences
Abstract
In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p = 0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac. (C) 2012 Elsevier Ltd. All rights reserved.
Keywords
Immunocastration; Tolbutamide; 7-benzyloxy-4-trifluoromethylcoumarin; 7-benzyloxyquinoline; CAR; PXR
Published in
Vaccine
2012, volume: 30, number: 24, pages: 3515-3518
Publisher: ELSEVIER SCI LTD
SLU Authors
Zamaratskaia, Galia
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Other Veterinary Science
Publication identifier
- DOI: https://doi.org/10.1016/j.vaccine.2012.03.072
Permanent link to this page (URI)
https://res.slu.se/id/publ/42476