Stimulatory effect of sesamin on hepatic cytochrome P450 activities in Atlantic salmon (Salmo salar L.) is not directly associated with expression of genes related to xenobiotic metabolism

Abstract

1. This study examined hepatic cytochrome P450 (CYP450) response to dietary sesamin in combination with different n-6/n-3 fatty acid ratios in fish diet. Over a period of 4 months, fish were fed seven different experimental diets an n-6/n-3 FA ratio of either 0.5 or 1.0 in combination with two sesamin levels: low sesamin = 1.16 g/kg feed and high sesamin = 5.8 g/kg feed. Control diets did not contain sesamin.2. The CYP450-associated activities of ethoxyresorufin O-deethylase (EROD), 7-benzyloxy-4-trifluoromethylcoumarin O-debenzylation (BFCOD), pentoxyresorufin O-depentylase (PROD), coumarin hydroxylase (COH), methoxyresorufin O-deethylase (MROD) and p-nitrophenol hydroxylase (PNPH) were significantly induced by dietary sesamin in a dose-related manner.3. Expressions of the genes CYP1A1, CYP1A3, CYP3A, AhR1 alpha, AhR2 beta, AhR2 delta and PXR involved in the regulation of CYP450 activities, was not the primary source of this induction.

Keywords

Fish; induction; xenobiotic metabolizing; enzymes

Published in

Xenobiotica
2015, volume: 45, number: 7, pages: 598-604
Publisher: INFORMA HEALTHCARE

SLU Authors

• Zlabek, Vladimir

  • University of South Bohemia in České Budějovice

• Schiller Vestergren, AnnaLotta

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Trattner, Sofia

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Wagner, Liane

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Pickova, Jana

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    
  • University of South Bohemia in České Budějovice

• Zamaratskaia, Galia

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    
  • University of South Bohemia in České Budějovice

UKÄ Subject classification

Molecular Biology
Biochemistry

Publication identifier

• DOI: https://doi.org/10.3109/00498254.2015.1007111

Permanent link to this page (URI)

https://res.slu.se/id/publ/68271