Sammanfattning

In this study, the relationships between post-thaw bull sperm characteristics and hyperketonemic conditions after coincubation with cow plasma or media were determined to investigate if such a condition could affect bull sperm characteristics. Two experiments were conducted. In experiment 1, blood samples were collected from 31 cows to prepare plasma. Cows were independently categorized into two groups according to plasma beta-hydroxybutyrate (BHB) concentrations (above or below 1.2 mM). Thawed bull semen was diluted and incubated with diluted plasma; motility parameters were evaluated using Computer Assisted Semen Analysis (CASA). In experiment 2, a pooled sample of thawed semen was diluted and divided into three aliquots: without BHB (control) and treated with either 1.2 mM (1.2) or 3 mM (3) BHB. In addition to motility, flow cytometric analyses were carried out. In experiment 1, the overall motility decreased significantly in plasma containing high (>= 1.2 mM) BHB compared to plasma containing low (<1.2 mM) BHB. In experiment 2, the overall motility tended to be lower in BHB (3 mM)-supplemented samples. The supplementation of 3 mM BHB increased the proportion of live superoxide-positive sperm and sperm with high mitochondrial potential, while the DNA fragmentation index decreased.

Nyckelord

bovine; coincubation; hyperketonaemia; semen characteristics

Publicerad i

Reproduction in Domestic Animals
2023, Volym: 58, nummer: 12, sidor: 1732-1744
Utgivare: WILEY

SLU författare

• Johannisson, Anders

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Morrell, Jane

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Klinisk vetenskap


Publikationens identifierare

DOI: https://doi.org/10.1111/rda.14492

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/126889