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Research article2008Peer reviewed

Molecular weight distribution and content of water-extractable beta-glucan in rye crisp bread

Andersson, A. A. M.; Ruegg, N.; Aman, P.

Abstract

Rye crisp doughs and breads were made from three rye flours with different falling numbers (i.e. different enzyme activity), with or without oat bran rich in beta-glucan (OBG) added, and with different fermentation times. The aim of the study was to investigate the effect of endogenous enzymes in the flour on molecular weight and content of water-extractable beta-glucan in the doughs and breads. The molecular weight distribution of beta-glucan was unimodal for the flours, with a calcofluor average molecular weight of about 90 x 10(4) g/mol for the rye flours, and 150 x 10(4) g/mol for rye flours with added OBG. The molecular weight decreased with increasing fermentation time for all doughs, but was almost unchanged during oven-baking. The calcofluor average molecular weight was highest in doughs and breads made of flour with highest falling number (i.e. lowest enzyme activity), indicating that the endogenous enzymes in the flour were responsible for the breakdown of beta-glucan. During fermentation, water-ex tractable beta-glucan was released from the insoluble matrix, probably by the endogenous enzymes in the flour. When the molecular weight of the water-extractable beta-glucan decreased, they probably associated with each other or with other components in the dough to form unextractable complexes. The content of waterextractable beta-glucan did not change during oven-baking. (C) 2007 Elsevier Ltd. All rights reserved.

Keywords

beta-glucan; rye; molecular weight; crisp bread; oats

Published in

Journal of Cereal Science
2008, Volume: 47, number: 3, pages: 399-406
Publisher: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD