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Research article - Peer-reviewed, 2012

Improvac does not modify the expression and activities of the major drug metabolizing enzymes cytochrome P450 3A and 2C in pigs

Tomankova, Jana; Rasmussen, Martin; Andersson, Kristina; Ekstrand, Bo; Zamaratskaia, Galia


In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p = 0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac. (C) 2012 Elsevier Ltd. All rights reserved.


Immunocastration; Tolbutamide; 7-benzyloxy-4-trifluoromethylcoumarin; 7-benzyloxyquinoline; CAR; PXR

Published in

2012, volume: 30, number: 24, pages: 3515-3518

Authors' information

Tomankova, Jana
Rasmussen, Martin
Aarhus University
Andersson, Kristina
Swedish University of Agricultural Sciences, Department of Animal Nutrition and Management
Ekstrand, Bo
Swedish University of Agricultural Sciences, Department of Food Science

UKÄ Subject classification

Other Veterinary Science

Publication Identifiers


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