Sun, Ren
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Research article2010Peer reviewed
Identification and Characterization of Mitochondrial Factors Modulating Thymidine Kinase 2 Activity
Sun, Ren; Eriksson, Staffan; Wang, Liya
Abstract
Thymidine kinase 2 (TK2) is one of four deoxynucleoside kinases (dNKs) in humans and plays a crucial role in the initial phosphorylation of pyrimidine nucleosides in the salvage pathway in mitochondria. Nucleoside analogues, like AZT, are substrates of TK2 and induced mitochondrial toxicity in long-term therapy. We found that AZT and FLT inhibited dThd phosphorylation but stimulated dCyd phosphorylation catalyzed by TK2. However, mitochondrial phosphorylation of both dThd and dCyd was inhibited by AZT and FLT. Here a preliminary identification and characterization of mitochondrial factors is reported.
Keywords
Mitochondrial thymidine kinase 2; AZT; FLT; mitochondrial factors
Published in
Nucleosides, Nucleotides and Nucleic Acids
2010, volume: 29, number: 4-6, pages: 382-385
Publisher: TAYLOR & FRANCIS INC
SLU Authors
Eriksson, Staffan
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Wang, Liya
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Veterinary Science
Biochemistry and Molecular Biology
Animal and Dairy Science
Publication identifier
- DOI: https://doi.org/10.1080/15257771003741018
Permanent link to this page (URI)
https://res.slu.se/id/publ/60548