Abstract

Thymidine kinase 2 (TK2) is one of four deoxynucleoside kinases (dNKs) in humans and plays a crucial role in the initial phosphorylation of pyrimidine nucleosides in the salvage pathway in mitochondria. Nucleoside analogues, like AZT, are substrates of TK2 and induced mitochondrial toxicity in long-term therapy. We found that AZT and FLT inhibited dThd phosphorylation but stimulated dCyd phosphorylation catalyzed by TK2. However, mitochondrial phosphorylation of both dThd and dCyd was inhibited by AZT and FLT. Here a preliminary identification and characterization of mitochondrial factors is reported.

Keywords

Mitochondrial thymidine kinase 2; AZT; FLT; mitochondrial factors

Published in

Nucleosides, Nucleotides and Nucleic Acids
2010, Volume: 29, number: 4-6, pages: 382-385
Publisher: TAYLOR & FRANCIS INC

SLU Authors

• Sun, Ren

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Eriksson, Staffan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Wang, Liya

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Veterinary Science
Biochemistry and Molecular Biology
Animal and Dairy Science


Publication identifier

DOI: https://doi.org/10.1080/15257771003741018

Permanent link to this page (URI)

https://res.slu.se/id/publ/60548