Newson, William
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Research article2017Peer reviewed
Effect of extraction routes on protein content, solubility and molecular weight distribution of Crambe abyssinica protein concentrates and thermally processed films thereof
Newson, William Roy; Prieto-Linde, Maria Luisa; Kuktaite, Ramune; Hedenqvist, Mikael S.; Gällstedt, Mikael; Johansson, Eva
Abstract
To understand if and how extraction conditions influence properties of molded protein films, Crambe abyssinica protein concentrates were produced from deoiled seed meal under various extraction condi-tions. Properties of the resulting hot compression molded films were evaluated through the molecular weight distribution, protein polymerization behavior and tensile tests. Precipitated protein concentrates demonstrated higher protein content and a pronounced shift to higher molecular weight distributions and reduced solubility on heating, indicating increased protein polymerization compared to those from lyophilized supernatants. Thermally processed films from isoelectrically precipitated prdtein concen-trates show a high resistance to extraction with a combination of reducing agent and denaturant, indicating the presence of non-disulfide covalent cross linking. Also, tensile strength was higher in con-centrates from precipitated proteins compared to those from supernatants. The protein concentrates resulting in thermally processed films with a high protein content, the highest levels of protein-protein interaction and high tensile strength were based on alkaline extraction and isoelectric precipitation. Therefore, a combination of alkali extraction and isoelectric precipitation is recommended to produce protein concentrates for molded film production. (C) 2017 Elsevier B.V. All rights reserved.
Keywords
Protein-based materials; Isoelectric precipitation; Compression molding; Industrial oilseed meal; Protein aggregation; Crambe abyssinica
Published in
Industrial Crops and Products
2017, Volume: 97, pages: 591-598
Publisher: ELSEVIER SCIENCE BV
Prieto-Linde, Maria Luisa
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Kuktaite, Ramune
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Johansson, Eva
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Department of Plant Breeding, Swedish University of Agricultural Sciences
SLU Authors
UKÄ Subject classification
Agricultural Science
Publication identifier
DOI: https://doi.org/10.1016/j.indcrop.2016.12.037
Permanent link to this page (URI)
https://res.slu.se/id/publ/80923