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Research article2017Peer reviewed

Effect of extraction routes on protein content, solubility and molecular weight distribution of Crambe abyssinica protein concentrates and thermally processed films thereof

Newson, William Roy; Prieto-Linde, Maria Luisa; Kuktaite, Ramune; Hedenqvist, Mikael S.; Gällstedt, Mikael; Johansson, Eva


To understand if and how extraction conditions influence properties of molded protein films, Crambe abyssinica protein concentrates were produced from deoiled seed meal under various extraction condi-tions. Properties of the resulting hot compression molded films were evaluated through the molecular weight distribution, protein polymerization behavior and tensile tests. Precipitated protein concentrates demonstrated higher protein content and a pronounced shift to higher molecular weight distributions and reduced solubility on heating, indicating increased protein polymerization compared to those from lyophilized supernatants. Thermally processed films from isoelectrically precipitated prdtein concen-trates show a high resistance to extraction with a combination of reducing agent and denaturant, indicating the presence of non-disulfide covalent cross linking. Also, tensile strength was higher in con-centrates from precipitated proteins compared to those from supernatants. The protein concentrates resulting in thermally processed films with a high protein content, the highest levels of protein-protein interaction and high tensile strength were based on alkaline extraction and isoelectric precipitation. Therefore, a combination of alkali extraction and isoelectric precipitation is recommended to produce protein concentrates for molded film production. (C) 2017 Elsevier B.V. All rights reserved.


Protein-based materials; Isoelectric precipitation; Compression molding; Industrial oilseed meal; Protein aggregation; Crambe abyssinica

Published in

Industrial Crops and Products
2017, Volume: 97, pages: 591-598