Functional Analysis of a Putative Dothistromin Toxin MFS Transporter Gene

Sammanfattning

Dothistromin is a non-host selective toxin produced by the pine needle pathogen Dothistroma septosporum. Dothistromin is not required for pathogenicity, but may have a role in competition and niche protection. To determine how D. septosporum tolerates its own toxin, a putative dothistromin transporter, DotC, was investigated. Studies with mutants lacking a functional dotC gene, overproducing DotC, or with a DotC-GFP fusion gene, did not provide conclusive evidence of a role in dothistromin efflux. The mutants revealed a major effect of DotC on dothistromin biosynthesis but were resistant to exogenous dothistromin. Intracellular localization studies suggest that compartmentalization may be important for dothistromin tolerance.

Nyckelord

aflatoxin biosynthesis; Dothistroma septosporum; major facilitator superfamily; toxin transporter; red-band needle blight

Publicerad i

Toxins
2009, Volym: 1, nummer: 2, sidor: 173-187

UKÄ forskningsämne

Skogsvetenskap


Publikationens identifierare

DOI: https://doi.org/10.3390/toxins1020173

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/84707