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Research article - Peer-reviewed, 2016

Determination of biogenic amines in aerobically stored beef using high-performance thin-layer chromatography densitometry

Li, S.; Johansson, M.; Vidanarachchi, J. K.; Pickova, J.; Zamaratskaia, G.


The aim of this study was to develop a high-performance thin-layer chromatography densitometry method to simultaneously evaluate levels of different biogenic amines (BAs) in beef stored aerobically at 4 degrees C. Steaks from M. longissimus thoracis et lumborum muscles were overwrapped with polyvinyl chloride film and stored for 0, 4, 7, and 10 days. For this purpose, the solvent system and pH for the derivatization of BAs with dansyl chloride were optimized. Moreover, this method was also validated for linearity, limits of detection and quantification, precision, and recovery. As found using this method in beef samples, spermine content decreased significantly after 7 days of storage, and tyramine and cadaverine were not detected until day 10.


Biogenic amine; HPTLC; beef spoilage

Published in

Acta Agriculturae Scandinavica, Section A - Animal Science
2016, Volume: 66, number: 4, pages: 199-205