Doxorubicin effects on leukemia and breast cancer cells in culture on the TK1 protein levels using AroCell TK 210 ELISA: a tool for drug development

Abstract

In this study changes in thymidine kinase 1 (TK1) levels after 24 hours of Doxorubicin (Dox) exposure of CEM and MDA MB-231 cells were determined using the commercially available AroCell TK 210 ELISA test. In cell extracts, TK1 levels increased twofold with 1 mu M Dox in both cell lines, while the TK1 levels in the culture media increased with 5 and 10 mu M of Dox only in case of CEM cells. In conclusion, this study reveals that the TK 210 ELISA can measure changes in intra- and extracellular TK1 levels apparently related to the mechanism of cytotoxicity of anti-cancer agents.

Keywords

Doxorubicin; TK 210 ELISA; Thymidine kinase 1

Published in

Nucleosides, Nucleotides and Nucleic Acids
2018, Volume: 37, number: 12, pages: 679-686

SLU Authors

• Jagarlamudi, Kiran Kumar

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Wang, Liya

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Eriksson, Staffan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

Sustainable Development Goals

SDG3 Good health and well-being


UKÄ Subject classification

Pharmacology and Toxicology


Publication identifier

DOI: https://doi.org/10.1080/15257770.2018.1478094

Permanent link to this page (URI)

https://res.slu.se/id/publ/99372