Determination of biogenic amines in aerobically stored beef using high-performance thin-layer chromatography densitometry

Abstract

The aim of this study was to develop a high-performance thin-layer chromatography densitometry method to simultaneously evaluate levels of different biogenic amines (BAs) in beef stored aerobically at 4 degrees C. Steaks from M. longissimus thoracis et lumborum muscles were overwrapped with polyvinyl chloride film and stored for 0, 4, 7, and 10 days. For this purpose, the solvent system and pH for the derivatization of BAs with dansyl chloride were optimized. Moreover, this method was also validated for linearity, limits of detection and quantification, precision, and recovery. As found using this method in beef samples, spermine content decreased significantly after 7 days of storage, and tyramine and cadaverine were not detected until day 10.

Keywords

Biogenic amine; HPTLC; beef spoilage

Published in

Acta Agriculturae Scandinavica, Section A - Animal Science
2016, Volume: 66, number: 4, pages: 199-205

SLU Authors

• Li, Shengjie

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    
  • Dalian Polytechnic University

• Johansson, Monika

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Pickova, Jana

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Zamaratskaia, Galia

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Other Veterinary Science


Publication identifier

DOI: https://doi.org/10.1080/09064702.2017.1315455

Permanent link to this page (URI)

https://res.slu.se/id/publ/90948