Research article - Peer-reviewed, 1998
Quantitative analysis of amylopectin unit chains by means of high-performance anion-exchange chromatography with pulsed amperometric detection
Andersson, Roger; Koch, Kristine; Åman, PerAbstract
High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD) is a widely used technique to study the chain length distribution of amylopectin. The chromatograms, however, do not directly reflect this distribution, since the PAD response changes with the degree of polymerization. In this study, waxy maize starch was debranched and fractionated on a Bio-Gel P-6 column and the response factors for maltosaccharides with DP 3–65 were determined. The detector response per μg glucan chains was shown to decrease considerably for DP 3–7 while the curve leveled out for DP larger than 15.
Keywords
Amylopectin; Starch; SaccharidesPublished in
Journal of Chromatography A1998, volume: 800, number: 2, pages: 199-206
Authors' information
Swedish University of Agricultural Sciences, Department of Food Science
Swedish University of Agricultural Sciences, Department of Food Science
Swedish University of Agricultural Sciences, Department of Food Science
UKÄ Subject classification
Food Science
Analytical Chemistry
Publication Identifiers
DOI: https://doi.org/10.1016/S0021-9673(97)01151-5
URI (permanent link to this page)
https://res.slu.se/id/publ/39610