Quantitative analysis of amylopectin unit chains by means of high-performance anion-exchange chromatography with pulsed amperometric detection

Andersson, Roger; Koch, Kristine; Åman, Per

doi:10.1016/S0021-9673(97)01151-5

Research article - Peer-reviewed, 1998

Quantitative analysis of amylopectin unit chains by means of high-performance anion-exchange chromatography with pulsed amperometric detection

Andersson, Roger; Koch, Kristine; Åman, Per

Abstract

High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC–PAD) is a widely used technique to study the chain length distribution of amylopectin. The chromatograms, however, do not directly reflect this distribution, since the PAD response changes with the degree of polymerization. In this study, waxy maize starch was debranched and fractionated on a Bio-Gel P-6 column and the response factors for maltosaccharides with DP 3–65 were determined. The detector response per μg glucan chains was shown to decrease considerably for DP 3–7 while the curve leveled out for DP larger than 15.

Keywords

Amylopectin; Starch; Saccharides

Published in

Journal of Chromatography A
1998, volume: 800, number: 2, pages: 199-206

Authors' information

Andersson, Roger

Swedish University of Agricultural Sciences, Department of Food Science

Koch, Kristine

Swedish University of Agricultural Sciences, Department of Food Science

Åman, Per

Swedish University of Agricultural Sciences, Department of Food Science

UKÄ Subject classification

Food Science
Analytical Chemistry

Publication Identifiers

DOI: https://doi.org/10.1016/S0021-9673(97)01151-5

URI (permanent link to this page)

https://res.slu.se/id/publ/39610